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Appl Environ Microbiol. 1974 October; 28(4): 696-699
Copyright © 1974 American Society for Microbiology. All Rights Reserved.
Center for Disease Control, Atlanta, Georgia 30333
ABSTRACT
Partitioned between aqueous methanol and petroleum ether, the unsaponifiable pigments of Mycobacterium kansasii were all epiphasic. Thin-layer chromatography of these carotenoids showed that M. kansasii formed at least nine pigments. These pigments were identified by their chromatographic properties and spectral characteristics as phytoene,
-carotene, neurosporene, lycopene, leprotene,
-carotene,
-carotene,
-carotene, and ß-carotene. Three additional pigmented spots on thin-layer chromatography found in trace amounts were possibly degradation products of the major carotenoids.
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