This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Gaustad, J. W. Articles by Hatcher, H. J. Search for Related Content PubMed Articles by Gaustad, J. W. Articles by Hatcher, H. J. Agricola Articles by Gaustad, J. W. Articles by Hatcher, H. J.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1974 November; 28(5): 748-752
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Test Method for the Evaluation of Virucidal Efficacy of Three Common Liquid Surface Disinfectants on a Simulated Environmental Surface

Research and Development, Economics Laboratory, Inc., St. Paul, Minnesota 55102

ABSTRACT

The Association of Official Analytical Chemists bacterial use-dilution test for evaluating liquid surface disinfectants was modified to determine the efficacy of three common environmental germicide compounds against representatives of four virus groups. Modifications were made to conform to the Environmental Protection Agency guidelines on virucidal testing procedures. Alterations included: the use of a concentrated tissue culture preparation of virus instead of a standard bacterial culture; HEp-2 tissue culture cells as a visible test system in place of standard nutrient broth; and controls to measure the virus titer end point and the toxicity of the disinfectant to the cell cultures. Comparison of control end points with results from the test proper were the measure of the effectiveness of the germicides against the viruses. Results are described which agree with those based on other methods previously reported.