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Appl Environ Microbiol. 1974 November; 28(5): 793-797
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Recovery of Heated Clostridium perfringens Type A Spores on Selective Media1

J. T. Barach, D. M. Adams and M. L. Speck

Department of Food Science, North Carolina State University, Raleigh, North Carolina 27607

ABSTRACT

The enumeration of Clostridium perfringens spores on sulfite-polymyxin-sulfadiazine agar (SPS), tryptone-sulfite-neomycin agar (TSN), Shahidi-Ferguson-perfringens agar (SFP), tryptone-sulfite-cycloserine agar (TSC), and TSN lacking antibiotics (BASE) was studied. The spores were heated at 105 to 120 C by the capillary-tube method. The media were about equally efficient for the enumeration of heat-activated spores. Efficiency of the media for the recovery of spores surviving heat treatments at ultrahigh temperatures varied as follows: TSC ≥ SFP > BASE > SPS > TSN. Greater recovery when survivors were enumerated on TSC or SFP was attributed to germination of injured spores by the lysozyme present in the egg yolk emulsion used in these media. Low recovery of survivors on TSN and SPS was due to both the absence of lysozyme and inhibition of injured spores by the selective agents of these media. Recovery of heated spores was reduced greatly by polymyxin, neomycin, and kanamycin, and slightly by sulfadiazine and D-cycloserine. The addition of lysozyme to SPS or TSN did not improve the percentage of heat-injured spores recovered because the selective agents of these media interfered with the action of lysozyme. The suitability of the selective media for the enumeration of survivors was greatly affected by the presence of certain foods.

FOOTNOTES

1 Paper No. 4420 of the Journal Series of the North Carolina Agricultural Experiment Station, Raleigh, N.C.

Appl Environ Microbiol. 1974 November; 28(5): 793-797
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





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Copyright © 1974 by the American Society for Microbiology. All rights reserved.