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Appl Environ Microbiol. 1974 November; 28(5): 802-806
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Microculture Plaque Neutralization Test for California Group Arboviruses

Gary L. Seawright1, Gherry Harding, Frederick C. Thomas2 and Robert P. Hanson

Department of Veterinary Science, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

A microculture plaque neutralization test is described for California-group arboviruses that is as precise and quantitative as the standard test conducted in 60-mm petri dishes. It was shown that there was no significant between-panel or between-day variation in determinations and that a single pipette could be used for all serum-dilution levels within a titration without inoculum carry-over effect. The experimental protocol and statistical methods used produce 50% neutralization end points that meet the assumptions of parametric statistics. This permits the power and versatility of the analysis of variance to be exploited in testing for treatment effects in serological and immunological studies with viruses.

FOOTNOTES

1 Present address: APHIS, Biologics Laboratories, P.O. Box 905, Ames, Iowa 50010.

2 Present address: Animal Pathology Division, Canada Department of Agriculture, Animal Disease Research Institute, P.O. Box 1400, Hull, P.Q., Canada.

Appl Environ Microbiol. 1974 November; 28(5): 802-806
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





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Copyright © 1974 by the American Society for Microbiology. All rights reserved.