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Appl Environ Microbiol. 1974 November; 28(5): 851-855
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Fluorescent Antibody Study of the Gram-Positive Anaerobic Cocci

Department of Microbiology and Immunology, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140

ABSTRACT

Fluorescent antibody conjugates were prepared from five species of anaerobic cocci commonly isolated from human infections. When tested with homologous and heterologous cells these conjugates were found to be highly specific. There was no evidence of a common genus antigen. Peptococcus magnus conjugates detected a species-specific antigen; cross-reactions with Peptostreptococcus anaerobius were readily eliminated by absorption. The conjugates from Peptococcus asaccharolyticus, Peptococcus prevotii, Peptostreptococcus, anaerobius, and Peptostreptococcus intermedius displayed a high degree of strain specificity. Occasional cross-reactions were detected with homologous strains, suggesting the presence of common antigens, but no attempt was made to determine the number of different serotypes in these species.