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Appl Environ Microbiol. 1974 November; 28(5): 889-891
Copyright © 1974 American Society for Microbiology. All Rights Reserved.
Agriculture Canada, Animal Diseases Research Institute (Western), Lethbridge, Alberta, Canada
ABSTRACT
Approximately 45-cm length of jejunoileum of 7-week-old chickens was found to be responsive and suitable for testing the enterotoxin of Clostridium perfringens by the ligated intestinal loop technique. Injections of 20 to 30 µg of enterotoxin per loop caused positive response of fluid accumulation. Chickens were found to be more convenient and economical for this purpose than other laboratory and domestic animals.
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