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Appl Environ Microbiol. 1974 December; 28(6): 1027-1029
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Further Characterization of Tissue Distribution and Metabolism of [¹⁴C]Aflatoxin B₁ in Chickens¹

Department of Poultry Science, Ohio State University, Columbus, Ohio 43210

ABSTRACT

The distribution and metabolism of [¹⁴C]aflatoxin B₁ in chicken tissues were further investigated. Previously dried and frozen ethyl acetate extracts of liver, heart, gizzard, breast, leg, blood, and fecal samples were obtained from either layer or broiler chickens fed subclinical levels of [¹⁴C]aflatoxin B₁. Treatment of these extracts with either carboxypeptidase A, leucine aminopeptidase, pepsin, or trypsin revealed that an average of 50% of the ¹⁴C detected in the acetate extracts was a liberated peptide (or amino acid) conjugate of [¹⁴C]aflatoxin B_2a. When a prepared standard of B_2a was made by incubation of B₁ with cold dilute aqueous HCl, the R_f values and absorbance maxima were identical with those of the tissue extracts after enzymatic treatment.

¹ Ohio Agricultural Research and Development Center Journal Article series no. 78-74.