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Appl Environ Microbiol. 1974 December; 28(6): 1030-1032
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Membrane Concentration of Infectious Bovine Rhinotracheitis Virus from Water¹

Departments of Bacteriology and Veterinary Science, North Dakota State University, Fargo, North Dakota 58102

ABSTRACT

A membrane adsorption procedure was used to concentrate infectious bovine rhinotracheitis virus from 1-liter quantities of distilled water. Cellulose nitrate membrane filters (0.45-µm pore size) efficiently adsorbed this herpesvirus from water, and virus was recovered from the membrane by elution with 10 ml of fetal calf serum during sonic treatment. The average recovery rate was 70%.

¹ Journal article 525 from the North Dakota Agricultural Experiment Station.