This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Arnold, W. N. Articles by Boyd, K. S. Search for Related Content PubMed Articles by Arnold, W. N. Articles by Boyd, K. S. Agricola Articles by Arnold, W. N. Articles by Boyd, K. S.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1974 December; 28(6): 1047-1054
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Periplasmic Structure in Saccharomyces rouxii (Boutroux), an Osmophil

Departments of Biochemistry and Microbiology, University of Kansas Medical Center, Kansas City, Kansas 66103, and Research Laboratory, Veterans Administration Hospital, Kansas City, Missouri 64128

ABSTRACT

Electron micrographs of ultrathin sections of S. rouxii displayed electrondense, membrane-circumscribed structures between the protoplasmic membrane and the cell wall. These periplasmic bodies were numerous in cells from a 3-day culture and absent or rare in older cells. Periplasmic bodies were fewer and smaller (flattened) in specimens grown in a medium fortified with 10% sucrose; they were not detected in cells grown in 20% sucrose. A brief treatment with ethyl acetate caused the periplasmic bodies of young cells to become electron light. Periplasmic bodies were most prevalent in the regions of the bud scars and were often accommodated within large invaginations in the protoplasmic membrane. In general, conditions which favor the prevalence and electron density of periplasmic bodies are those which also mask the activity of ß-fructofuranosidase in this species.