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Appl Environ Microbiol. 1975 February; 29(2): 170-174
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Polarographic Assay of Hydrogen Peroxide Accumulation in Microbial Cultures

Dairy and Food Microbiology Department, University College, Cork, Ireland

ABSTRACT

A method is described for determining low concentrations of hydrogen peroxide by using a polarographic oxygen electrode to measure the oxygen released into solution on addition of catalase. A sample can be assayed directly without prior manipulation in 3 min. The method is capable of assaying hydrogen peroxide concentrations as low as 7 µM. The method has proved extremely useful for the assay of hydrogen peroxide secreted into milk by lactic acid bacteria.

¹ Present address: Department of Animal Sciences, University of Kentucky, Lexington, Ky.