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Appl Environ Microbiol. 1975 February; 29(2): 300-302
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Rapid Method for Preparing Cell-Free Extracts of Aspergillus ochraceus

Department of Botany and Microbiology, Auburn University, Agricultural Experiement Station, Auburn, Alabama 36830

ABSTRACT

A rapid method for preparing cell-free extracts of Aspergillus ochraceus was developed. Mycelial mats were prefrozen in liquid nitrogen, ground to a fine powder in a cold mortar, and homogenized in an all-glass mechanical homogenizer. This method provided preparations averaging 43.0 mg of protein per g of mycelium (wet weight). The method was fast, efficient, and did not subject the extract to temperatures above 1 C or to heavy metals. The preparation method was suitable for studying a variety of in vitro fungal enzyme systems. Amylase, acid phosphatase, alkaline phosphatase, catalase, fatty acid synthetase, glucose-6-phosphate dehydrogenase, beta-glucosidase, beta fructofuranosidase, and trehalase activities were measurable in the preparations.

¹ Present address: Commercial Solvents Corp., Research and Development Div., Terre Haute, Ind. 47808.