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Appl Environ Microbiol. 1975 March; 29(3): 323-327
Copyright © 1975 American Society for Microbiology. All Rights Reserved.
Northern Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604
ABSTRACT
A laboratory-scale fermentor designed for solid-substrate fermentation was constructed and tested. Its capacity to produce ochratoxin under varied conditions was determined with wheat as substrate. Ochratoxin yields of 2,000 to 2,500 µg/g of wheat were regularly obtained, and occasionally yields as high as 4,000 µg/g were obtained. The most critical factor in the fermentation was initial substrate moisture content; wheat tempered at 30 to 31% moisture produced the highest yields. Other variables tested were agitation and aeration rates, initial static culture time, and inoculum types and volumes.
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