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Appl Environ Microbiol. 1975 March; 29(3): 422-426
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Powdered Chitin Agar as a Selective Medium for Enumeration of Actinomycetes in Water and Soil¹

^* Bureau of Laboratories, Michigan Department of Public Health, Lansing, Michigan 48914, and Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824

ABSTRACT

Agar media made with 0.4% colloidal chitin plus mineral salts and adjusted to pH 8.0 was superior to four other commonly used media for the isolation and enumeration of actinomycetes from water samples. More actinomycetes developed on chitin agar, and the development of bacteria and fungi was suppressed. Frozen and vacuum-dried chitin from aqueous colloidal suspensions was finely divided and gave results comparable to those obtained with media prepared from colloidal suspensions.

¹ Journal article no. 7025 from the Michigan Agricultural Experiment Station.