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Appl Environ Microbiol. 1975 April; 29(4): 469-471
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Effects of Bicarbonate on Growth of Neisseria gonorrhoeae: Replacement of Gaseous CO₂ Atmosphere

Department of Biological Sciences, Texas Tech University, Lubbock, Texas 79409

ABSTRACT

The effect of NaHCO₃ on the growth of Neisseria gonorrhoeae cultures was studied in a liquid and a semisolid growth medium. With a broth culture, NaHCO₃ (0.009 M) greatly reduced the lag phase and also increased the total growth. The same concentration of bicarbonate supported rapid growth when added to the semisolid medium if the plates were individually incubated in sealed plastic bags. In a container with a large air space, a higher concentration of NaHCO₃ was necessary to support growth. The assimilation of ¹⁴C-labeled NaHo₃ by growing cultures was also investigated.