This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Caputo, R. A. Articles by Farrell, M. P. Search for Related Content PubMed Articles by Caputo, R. A. Articles by Farrell, M. P. Agricola Articles by Caputo, R. A. Articles by Farrell, M. P.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1975 April; 29(4): 476-479
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Rapid Determination of the Amount of Cetylpyridinium Chloride Bound by Bacteria

Departments of Microbiology,^* Chemistry, and Zoology and Academic Computer Services, Miami University, Oxford, Ohio 45056

ABSTRACT

A modification of the Colowick and Womack procedure for measuring ligand binding by macromolecules is described for drug binding by bacteria. This technique is based on the determination of drug concentration in the dialysate from a bacteria-drug mixture at equilibrium. The dialysis cell of the original procedure was replaced by a Minibeaker (Bio-Rad), which has a larger membrane surface area, and the dialysate was continuously monitored with a spectrophotometer equipped with a flow cell. With this system, only 3 min was required to determine the amount of cetylpyridinium chloride bound by Escherichia coli K-12 strain P678. Possible applications of the technique are discussed.