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Appl Environ Microbiol. 1975 May; 29(5): 610-614
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Growth Curves of Anaerobic Bacteria in Solid Media

Infectious Disease Section, Veterans Administration, Wadsworth Hospital Center, Los Angeles, California 90073

ABSTRACT

Simple pour plate and spectrophotometric techniques for the evaluation of growth curves of several anaerobic bacteria on solid media are described. Three basic patterns of anaerobic growth were observed. The curves obtained were very reproducible when studied on separate occasions. The curves obtained by spectrophotometric measurement were comparable to those obtained by the pour plate method, especially when a large bacterial inoculum was used. Limitations in the interpretation of the results are discussed. The methods and principles reported could provide the basis for the determination of bacterial growth on solid media using other organisms and different experimental conditions.