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Appl Environ Microbiol. 1975 May; 29(5): 653-657
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Qualitative and Quantitative Assay of Trichothecin: a Mycotoxin Produced by Trichothecium roseum

Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73069

ABSTRACT

A method for quantitative determination of trichothecin in crude culture filtrates was presented. The method utilized an agar diffusion bioassay against Candida albicans, a colorimetric test involving a halochromatic reaction with sulfuric acid, and subsequent formation of blue color with methanol, and thin-layer chromatography of trichothecin and its dinitrophenylhydrazine derivative. A positive result in all three systems confirmed the presence of trichothecin. Quantitative results were generally in close agreement.