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Appl Environ Microbiol. 1975 August; 30(2): 298-308
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Influence of Substrate Wettability on the Attachment of Marine Bacteria to Various Surfaces¹

Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543

ABSTRACT

The effect of the initial substrate surface condition, as indicated by the critical surface tension for wetting, on the rate of attachment of marine bacteria to a variety of solid surfaces has been measured. The techniques used to determine the number of bacteria attached per unit surface area were a lipopolysaccharide test utilizing Limulus lysate and direct examination of the surface by scanning electron microscopy. The results obtained by the two techniques are compared and their significance to the control of microbiological slime film formation (microfouling) is discussed.

¹ Contribution no. 3523 from the Woods Hole Oceanographic Institution, Woods Hole, Mass. 02543.