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Appl Environ Microbiol. 1975 October; 30(4): 685-691
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Optimum Membrane Structures for Growth of Coliform and Fecal Coliform Organisms

Millipore Corporation, Beaford, Massachusetts 01730

ABSTRACT

The purpose of this study was to determine the optimum membrane filter structure and characteristics for recovery of coliform organisms. Additionally, other factors such as sterilization method and membrane composition were examined. Fecal coliform growth tests with varied samples indicated that the most critical factor in recovery was surface pore morphology and not other factors previously suspected. Fecal coliform counts showed a dramatic increase, with increasing surface opening sizes. Membrane structures with surface openings large enough to surround the entrapped bacteria are required for optimum growth of fecal coliform organisms. Maximum fecal coliform recoveries are obtained using membranes composed of mixed esters of cellulose exhibiting a surface opening diameter of 2.4 µm and a retention pore size of 0.7 µm.