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Appl Environ Microbiol. 1975 October; 30(4): 704-706
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Bacteriological Evaluation of an Ultra-Pure Water-Distilling System

Distillation Research Laboratory, Rochester Institute of Technology, Rochester, New York 14614; and Phoenix Laboratories Division, Center for Disease Control, Phoenix, Arizona 85014

ABSTRACT

A prototype distillation and storage system with recycle for producing ultrapure water was monitored for bacteriological contamination during a period of 24 months. Naturally occurring Pseudomonas aeruginosa and P. cepacia were found to grow rapidly to levels of about 10⁵/ml in water taken from the storage reservoir and also in commercially prepared distilled water. The system was found to eliminate bacterial contaminants introduced into the still with the feed water, but the reservoir, once contaminated, remained contaminated during prolonged recycle. After a single treatment with free chlorine, the entire system remained uncontaminated until accidental or purposeful shutdown.