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Appl Environ Microbiol. 1975 November; 30(5): 750-754
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

D-Glucose Isomerase: Constitutive and Catabolite Repression-Resistant Mutants of Streptomyces phaeochromogenes

Instituto de Investigaciones Biomédicas, Departamento de Biotecnología, Universidad Nacional Autónoma de México, México 20, D.F.

ABSTRACT

As in other Streptomyces species, the enzymatic conversion of D-glucose to D-fructose is carried out in Streptomyces phaeochromogenes NRRL B-3559 by the inducible enzyme, D-xylose keto isomerase (EC 5.3.1.5.). Mutants of this microorganism were selected for their ability to grow on D-lyxose (2-epimer of D-xylose). As a result of the mutational event, the microorganism constitutively produced D-xylose isomerase. As in the parent strain, the constitutive formation of the isomerase was repressed by D-glucose. The fact that this mutant was unable to grow in low D-xylose concentrations in the presence of the D-glucose analogue, 3-O-methylglucose, permitted the isolation of D-xylose isomerase constitutive mutants which were insensitive to D-glucose repression.