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Appl Environ Microbiol. 1975 November; 30(5): 825-830
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Development of a Micromethod for Identification of Anaerobic Bacteria

Anaerobe Laboratory, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

ABSTRACT

A microprocedure was described for determining the carbohydrate fermentation patterns of 48 anaerobic bacteria at one time in microtiter plates. The cultures were transferred into agar-filled wells of microtiter plates with a replicator inside an anaerobic glove box. Fermentation was measured both with a colorimetric indicator and with a small pH electrode. The method was approximately 97% accurate. It would be most useful for laboratories that need to identify large numbers of anaerobes at one time.