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Appl Environ Microbiol. 1976 March; 31(3): 395-398
Copyright © 1976 American Society for Microbiology. All Rights Reserved.

Growth Requirements of San Francisco Sour Dough Yeasts and Bakers' Yeast

¹ Western Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Berkeley, California 94710

ABSTRACT

The growth requirements of several yeasts isolated from San Francisco sour dough mother sponges were compared with those of bakers' yeast. The sour dough yeasts studied were one strain of Saccharomyces uvarum, one strain of S. inusitatus, and four strains of S. exiguus. S. inusitatus was the only yeast found to have an amino acid requirement, namely, methionine. All of the yeasts had an absolute requirement for pantothenic acid and a partial requirement for biotin. Inositol was stimulatory to all except bakers' yeast. All strains of S. exiguus required niacin and thiamine. Interestingly, S. inusitatus, the only yeast that required methionine, also needed folic acid. For optimal growth of S. exiguus in a molasses medium, supplementation with thiamine was required.