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Appl Environ Microbiol. 1977 March; 33(3): 630-634
Copyright © 1977 American Society for Microbiology. All Rights Reserved.
a Department of Bacteriology, Iowa State University, Ames, Iowa 50011
ABSTRACT
A new method was developed for the detection of injured and uninjured salmonellae in foods and feeds. The steps of pre-enrichment in a nonselective broth and selective enrichment in a selective medium were combined into a single procedure. This was achieved by the gradual release of selective agents from wax-coated gelatin capsules added at the time of inoculation of nonselective basal broths. Pre-enrichment in lactose broth was combined with selective enrichment in tetrathionate or selenite-cystine broth by using timed-release capsules containing iodine or selenite. Five different categories of foods and feeds, naturally contaminated with salmonellae, were examined to compare the efficiencies of the capsule methods with conventional procedures. Combination of the separate steps of pre-enrichment and selective enrichment into a single procedure was feasible and resulted in substantial savings of labor and materials.
2 Present address: Department of Food Technology, Iowa State University, Ames, IA 50011.
1 Journal paper no. J-8634 of the Iowa Agriculture and Home Economics Experiment Station, Ames; project no. 2018.
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