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Appl Environ Microbiol. 1977 March; 33(3): 718-724
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Characterization of a Cytoplasmic Reserve Glucan from Ruminococcus albus

K.-J. Cheng*, R. G. Brown and J. W. Costerton

Research Station, Agriculture Canada, Lethbridge, Alberta, Canada T1J 4B1
Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4H7
Department of Biology, University of Calgary, Calgary, Alberta, Canada T2N 1N4

ABSTRACT

Ruminococcus albus, an anaerobic bacterium that digests cellulose in the rumen of cattle, produces intracellular polysaccharide granules varying from 0.05 to 0.31 µm in diameter when grown in batch culture. This polysaccharide material was purified and found to contain D-glucose as the only reducing sugar. The polyglucose polymer was slightly opalescent in aqueous solution and formed a strong reddish purple iodine complex with a maximum absorbance at 550 nm. Its infrared spectrum had characteristic absorption bands at 8.70, 9.25, and 9.75 µm and was identical with that of the amylopectin-glycogen type of Megasphaera elsdenii and that of the glycogen of enteric bacteria and beef liver. It reacted strongly with concanavalin A. Methylation analysis showed that the glucan contained 2,3,4,6-tetra-O-MeG-2,3,6-Tri-O-MeG-2,3-Di-O-MeG in a ratio of 8:84:8. Characterization of the products obtained by treatment with isoamylase indicates that the glucan of R. albus is of the glycogen type.

Appl Environ Microbiol. 1977 March; 33(3): 718-724
Copyright © 1977 American Society for Microbiology. All Rights Reserved.



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