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Appl Environ Microbiol. 1977 April; 33(4): 878-880

Bioassay for homogeneous parasporal crystal of Bacillus thuringiensis using the tobacco hornworm, Manduca sexta.

J H Schesser, K J Kramer and L A Bulla Jr

ABSTRACT

A method for determining the toxicity of Bacillus thuringiensis subsp. kurstaki parasporal crystal to the tabocco hornworm, Manduca sexta, is described. The use of both mortality and weight loss data have provided a highly sensitive and reproducible bioassay that can be used to compare relative toxicities of crystals from other subspecies as well as toxic components contained therein.

Appl Environ Microbiol. 1977 April; 33(4): 878-880




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