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Appl Environ Microbiol. 1977 October; 34(4): 419-423
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Bile Salt Degradation by Nonfermentative Clostridia

David E. Mahony1, C. Elizabeth Meier1, Ian A. Macdonald1 and Lillian V. Holdeman2

1 Departments of Microbiology and Medicine, Dalhousie University, Halifax, Nova Scotia, Canada
2 The Anaerobe Laboratory, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24060

ABSTRACT

Eight strains of nonfermentative clostridia were characterized on the basis of their intracellular nicotine adenine dinucleotide- and nicotinamide adenine dinucleotide phosphate-dependent hydroxysteroid dehydrogenase (HSDH) content, ability to deconjugate taurocholate, growth characteristics, and metabolic products, including utilization of lactate and pyruvate. Two cultures of Clostridium sporosphaeroides (representing one strain obtained from two different sources), one strain of Clostridium irregularis, four strains of an unnamed species (Clostridium group SPH-1), and one strain of an unnamed species (Clostridium group P) were studied. Both cultures of C. sporosphaeroides contained low amounts of 7{alpha}-HSDH; C. irregularis contained only a low amount of 3{alpha}-HSDH. All four strains of Clostridium SPH-1 contained both 12{alpha}- and 7{alpha}-HSDH in the ratio of approximately 10:1. The strain of Clostridium group P contained only 12{alpha}-HSDH and was devoid of any other bile salt oxidoreductases. The enzyme preparation from Clostridium group P was useful in spectrophotometric quantitative studies of 12{alpha}-OH groups. Correlation of bile salt degradative activities with other phenotypic tests for characterization of and differentiation among such organisms is discussed.

Appl Environ Microbiol. 1977 October; 34(4): 419-423
Copyright © 1977 American Society for Microbiology. All Rights Reserved.





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