This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Linehan, B. Articles by Wolin, M. J. Search for Related Content PubMed PubMed Citation Articles by Linehan, B. Articles by Wolin, M. J. Agricola Articles by Linehan, B. Articles by Wolin, M. J.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1978 February; 35(2): 317-322
Copyright © 1978 American Society for Microbiology. All Rights Reserved.

Nutritional Requirements of Selenomonas ruminantium for Growth on Lactate, Glycerol, or Glucose

B. Linehan^,a, C. C. Scheifinger^,a and M. J. Wolin¹

¹ Division of Laboratories and Research, New York State Department of Health, Albany, New York, 12201
^a Department of Dairy Science and Microbiology, University of Illinois, Urbana, Illinois 61801

ABSTRACT

The nutritional requirements of Selenomonas ruminantium HD4 for growth on glucose, glycerol, or lactate were investigated to clarify the results of previous studies and to relate the nutrition of the organism to its physiology. The organism required L-aspartate, CO₂, p-aminobenzoic acid, and biotin for growth on a lactate-salts medium that contained small amounts of dithiothreitol. Aspartate could be replaced by L-malate or fumarate but not by succinate or L-asparagine. Requirements for growth with glycerol as an energy source were similar, except that aspartate was not required. With glucose as the energy source, neither aspartate nor p-aminobenzoic acid was required, but a requirement for volatile fatty acids, which could be met by n-valerate, was observed. CO₂ was required for growth on lactate or glycerol but not on glucose on complex media containing Trypticase and yeast extract. Sulfide could be used as the sole source of sulfur.

Present address: c/o Kevin Nedrow, Clifton Springs, NY 14432.

Present address: Greenfield Laboratories, Eli Lilly, Greenfield, IN 46140.

This article has been cited by other articles:

• Yamaguchi, Y., Takatsuka, Y., Matsufuji, S., Murakami, Y., Kamio, Y. (2006). Characterization of a Counterpart to Mammalian Ornithine Decarboxylase Antizyme in Prokaryotes. J. Biol. Chem. 281: 3995-4001 [Abstract] [Full Text]