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Appl Environ Microbiol. 1978 February; 35(2): 376-382
Copyright © 1978 American Society for Microbiology. All Rights Reserved.

Rapid Determination of Bacteriological Water Quality by Using Limulus Lysate

T. M. Evans, J. E. Schillinger and D. G. Stuart

¹ Department of Microbiology, Montana State University, Bozeman, Montana 59717

ABSTRACT

The Limulus lysate assay was used to measure the endotoxin content in stream water and was found to reflect the degree of bacterial contamination as measured by coliform, enteric, gram-negative, and heterotrophic bacteria. The firm-clot method was found to be a less sensitive and reproducible technique for the detection of endotoxin than was the spectrophotometric modification of the Limulus lysate assay. Bound endotoxin, as determined by the spectrophotometric modification of the Limulus lysate assay, was found to be a better measure of the endotoxin associated with bacterial cells than was total endotoxin.

Present address: Department of Microbiology, University of New Hampshire, Durham, NH 03824.

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