This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sacks, L E Articles by Thompson, P A Search for Related Content PubMed PubMed Citation Articles by Sacks, L E Articles by Thompson, P A Agricola Articles by Sacks, L E Articles by Thompson, P A

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1978 February; 35(2): 405-410

Clear, defined medium for the sporulation of Clostridium perfringens.

ABSTRACT

A new, defined medium for the sporulation of Clostridium perfringens is presented. Sporulation levels exceeding 10(6) to 10(7) heat-resistant spores per ml were obtained for seven strains: PS49, PS52, FD-1, T-65, NCTC strains 8798, 8238, and 10240. In the presence of theophylline, a methylxanthine, higher levels of heat-resistant spores were attained for strains PS49, PS52, FD-1, ant T-65; photomicrographs demonstrated a higher fraction of sporulating cells when these strains were grown in the presence of methylxanthines. Use of washed, highly diluted (less than 100 cells) inocula resulted in no reduction in spore yield. Strain KA3 grew well but sporulated poorly on this medium. The medium was clear and free of precipitate when small amounts (100 microgram/ml) of methylxanthine were incorporated.

This article has been cited by other articles:

• Orsburn, B., Melville, S. B., Popham, D. L. (2008). Factors Contributing to Heat Resistance of Clostridium perfringens Endospores. Appl. Environ. Microbiol. 74: 3328-3335 [Abstract] [Full Text]