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Appl Environ Microbiol. 1978 February; 35(2): 450-452
Copyright © 1978 American Society for Microbiology. All Rights Reserved.

Method for Adenosine 5'-Triphosphate Measurement on Coke Waste Activated Sludge

James Russell and Joseph J. Gauthier

Department of Biology, University of Alabama in Birmingham, Birmingham, Alabama 35294

ABSTRACT

Measurement of adenosine 5'-triphosphate (ATP) in coke waste activated sludge can provide a simple method for estimating the levels of viable microbes in the sludge. However, the presence of inhibitors such as phenol in the sludge interferes when the luciferin-luciferase method is used to measure ATP. These inhibiting substances can be removed from the sludge before extraction of ATP by washing the cells with dilute sodium dodecyl sulfate.

Appl Environ Microbiol. 1978 February; 35(2): 450-452
Copyright © 1978 American Society for Microbiology. All Rights Reserved.





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Copyright © 1978 by the American Society for Microbiology. All rights reserved.