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Appl Environ Microbiol. 1978 July; 36(1): 139-143
Copyright © 1978 American Society for Microbiology. All Rights Reserved.

Denitrification Rates in a Marine Sediment as Measured by the Acetylene Inhibition Technique

Institute of Ecology and Genetics, University of Aarhus, Ny Munkegade, DK-8000 Aarhus C, Denmark

ABSTRACT

A method has been developed for measurement of denitrification activity in sediments by application of the acetylene inhibition technique. Acetylene-saturated water was injected, at close intervals, into sediment cores which were then incubated for a few hours at the in situ temperature. Frozen segments of the cores were assayed for accumulation of N₂O by a combined gas extraction and detection system. The segments were thawed under a stream of helium from which N₂O (and other gases) was collected in a liquid N₂ trap, and the quantity of N₂O was measured by gas chromatography. The maximum rate of denitrification in a coastal marine sediment was 35 nmol of N per cm³ of sediment per day at 2.5°C, and the rate of denitrification for the total sediment was 0.99 nmol of N per m² per day.

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