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Appl Environ Microbiol. 1980 February; 39(2): 466-469

Density Centrifugation Method for Recovering Rhizobium spp. from Soil for Fluorescent-Antibody Studies

¹ Departments of Soil Science and Agronomy, North Carolina State University, Raleigh, North Carolina 27650
² The Ohio State University and Ohio Agricultural Research and Development Center, Columbus, Ohio 43210

ABSTRACT

A density centrifugation procedure has been developed as a replacement for soil flocculation and clarification steps employed in quantitative fluorescent-antibody studies on Rhizobium in soils. Near-quantitative recovery of added cells of two strains of Rhizobium japonicum and two strains of R. phaseoli was achieved from six soils with various properties. It is proposed that this technique may prove useful in separating other soil microorganisms from soil particles in ecological studies employing fluorescent-antibody techniques.

Published with permission of the Director, Ohio Agricultural Research and Development Center, as Journal article no. 149-79.