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Appl Environ Microbiol. 1980 April; 39(4): 900-907

Polyacrylamide Slab Gel Electrophoresis of Soluble Proteins for Studies of Bacterial Floras

W. E. C. Moore, D. E. Hash, Lillian V. Holdeman and Elizabeth P. Cato

1 Anaerobe Laboratory, College of Agriculture and Life Sciences, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

ABSTRACT

A polyacrylamide slab gel electrophoresis procedure was used to compare cellular proteins from bacterial isolates of gingival crevice floras. Isolates with identical protein patterns consistently were shown to be members of the same species. When used to screen isolates, the procedure reduced total analytical time and expense without sacrificing accuracy, and it provided additional verification of the identity of strains characterized by conventional phenotypic tests.


Appl Environ Microbiol. 1980 April; 39(4): 900-907




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