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Appl Environ Microbiol. 1980 May; 39(5): 1042-1045

Fluorescent Staining Technique for Nucleoid Regions of Streptosporangium albidum and Streptosporangium brasiliense

J. L. Speth and C. H. Nash

¹ Research and Development Division, Smith Kline & French Laboratories, Philadelphia, Pennsylvania 19101

ABSTRACT

Fluorescent staining procedures were developed for elucidating the nucleoid region in Streptosporangium albidum and Streptosporangium brasiliense. In these procedures, plugs of nutrient agar were inoculated with the microorganims and then covered with a sterile glass slide. The growing cells adhered to the surface of the slide and remained attached throughout the staining procedures. Two separate staining methods were utilized, one with bisbenzimid H33258 and the other with auramine O. Fluorescent microscopy revealed intensely stained nucleoid regions within mycelia, spores, and sporangia.

Present address: Medical Research Division, Lederle Laboratories, Pearl River, NY 10965.