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Appl Environ Microbiol. 1981 January; 41(1): 255-260

Extraction of rotavirus from human feces by treatment with lithium dodecyl sulfate.

M C Croxson and A R Bellamy

ABSTRACT

A procedure has been developed for the isolation of rotavirus from human fecal specimens based on the resistance of the virus to treatment with cold 1% lithium dodecyl sulfate at neutral pH. A single detergent treatment of fecal material followed by low- and high-speed centrifugations yielded a virus suspension of sufficient purity for viral ribonucleic acid to be analyzed directly by electrophoresis on polyacrylamide gels.

Appl Environ Microbiol. 1981 January; 41(1): 255-260






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