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Appl Environ Microbiol. 1981 January; 41(1): 60-70

Inorganic Carbon Limitation and Chemical Composition of Two Freshwater Green Microalgae

Joel C. Goldman and Stephen J. Graham

Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543

ABSTRACT

Two freshwater chlorophytes, Chlorella vulgaris and Scenedesmus obliquus, were grown in inorganic carbon-limited continuous cultures in which HCO₃^– was the sole source of inorganic carbon. The response of the steady-state growth rate to the external total inorganic carbon concentration was reasonably well described by the Monod equation; however, the response to the internal nutrient concentration was only moderately well represented by the Droop equation when the internal carbon concentration was defined on a cellular basis. The Droop equation was totally inapplicable when total biomass (dry weight) was used to define internal carbon because the ratio of carbon to dry weight did not vary over the entire growth rate spectrum. In batch cultures, maximum growth rates were achieved at the CO₂ levels present in atmospheric air and at HCO₃^– concentrations of 3 mM. No growth was observed at 100% CO₂. Both nitrogen uptake and chlorophyll synthesis were tightly coupled to carbon assimilation, as indicated by the constant C/N and C/chlorophyll ratios found at all growth rates. The main influence of inorganic carbon limitation appears to be not on the chemical structure of the biomass, but rather on cell size; higher steady-state growth rates lead to bigger cells.

Present address: Division of Applied Sciences, Harvard University, Cambridge, MA 02138.

Contribution 4658 from the Woods Hole Oceanographic Institution.