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Appl Environ Microbiol. 1981 February; 41(2): 396-403

1 Water Resources Division, U.S. Geological Survey, Menlo Park, California 94025
2 Department of Civil and Environmental Engineering, Stanford University, Stanford, California 94025
ABSTRACT
Acetylene disappeared from the gas phase of anaerobically incubated estuarine sediment slurries, and loss was accompanied by increased levels of carbon dioxide. Acetylene loss was inhibited by chloramphenicol, air, and autoclaving. Addition of 14C2H2 to slurries resulted in the formation of 14CO2 and the transient appearance of 14C-soluble intermediates, of which acetate was a major component. Acetylene oxidation stimulated sulfate reduction; however, sulfate reduction was not required for the loss of C2H2 to occur. Enrichment cultures were obtained which grew anaerobically at the expense of C2H2.
Present address: Federal Institute for Water Resources and Pollution Control, Swiss Federal Institute of Technology, CH-8500, Dübendorf, Switzerland.
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