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Appl Environ Microbiol. 1981 February; 41(2): 430-436

Production of Ethanol from D-Xylose by Using D-Xylose Isomerase and Yeasts

Laboratory of Renewable Resources Engineering, A. A. Potter Engineering Center, Purdue University, West Lafayette, Indiana 47907

ABSTRACT

D-Xylulose, an intermediate of D-xylose catabolism, was observed to be fermentable to ethanol and carbon dioxide in a yield of greater than 80% by yeasts (including industrial bakers' yeast) under fermentative conditions. This conversion appears to be carried out by many yeasts known for D-glucose fermentation. In some yeasts, xylitol, in addition to ethanol, was produced from D-xylulose. Fermenting yeasts are also able to produce ethanol from D-xylose when D-xylose isomerizing enzyme is present. The results indicate that ethanol could be produced from D-xylose in a yield of greater than 80% by a two-step process. First, D-xylose is converted to D-xylulose by xylose isomerase. D-Xylulose is then fermented to ethanol by yeasts.

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