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Appl Environ Microbiol. 1981 April; 41(4): 1043-1045

Detection of Rotavirus in Sewage

¹ Department of Medical Microbiology, University of Kiel, 2300 Kiel 1, West Germany

ABSTRACT

For detection of rotavirus, domestic sewage was concentrated by two different methods: (i) adsorption to and elution from positively charged Seitz filters, followed by ultracentrifugation, and (ii) chemical precipitation. The concentrated fluids were tested by an enzyme-linked immunosorbent assay and electron microscopy. In 6 of 24 (25%) samples, rotavirus was detectable after the combined filtration and ultracentrifugation technique with both an enzyme-linked immunosorbent assay and electron microscopy. No positive results were obtained after chemical precipitation.

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