This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mattiasson, B. Articles by Mosbach, K. Search for Related Content PubMed PubMed Citation Articles by Mattiasson, B. Articles by Mosbach, K. Agricola Articles by Mattiasson, B. Articles by Mosbach, K.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1981 April; 41(4): 903-908

Enzyme Thermistor Analysis of Penicillin in Standard Solutions and in Fermentation Broth

Pure and Applied Biochemistry, Chemical Center, University of Lund, S-220 07 Lund 7, Sweden

ABSTRACT

Immobilized penicillinase was applied in an enzyme thermistor for calorimetric analysis of samples containing penicillin G. Standard solutions as well as extracts from fermentation broth were analyzed. The enzyme was applied bound either to porous glass or, when dealing with crude preparations, to the inner surface of nylon tubing. In the fermentation system studied, high concentrations of penicillin were present, thus allowing dilution to reduce the influence of the composition of the medium on the analysis. The useful linear concentration range was from 0.1 to 100 mM. The coefficient of correlation between analytical results obtained with the present method and those from conventional assays was 0.997.