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Appl Environ Microbiol. 1981 August; 42(2): 370-374

Effects of Phosphate in Medium on Protein Secretion in a Protein-Producing Bacterium, Bacillus brevis 47

Norihiro Tsukagoshi, Hidehiko Yamada, Akio Tsuboi and Shigezo Udaka

Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Nagoya, Japan

ABSTRACT

Bacillus brevis 47 secreted up to 1 mg of protein per ml in a chemically defined medium, depending on phosphate concentration. The composition of exoproteins was altered quantitatively by the concentration of external phosphate. Morphologically, B. brevis 47 showed a distinct three-layered cell wall structure and shed the outer two layers during growth.

Appl Environ Microbiol. 1981 August; 42(2): 370-374






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