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Appl Environ Microbiol. 1981 August; 42(2): 385-387

Toxicity of Bacillus thuringiensis Spo^– Cr⁺ Mutants for the European Corn Borer Ostrinia nubilalis

¹ Northern Regional Research Center, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Peoria, Illinois 61604

ABSTRACT

Inclusion bodies isolated from Spo^– Cr⁺ mutants of Bacillus thuringiensis were toxic for larvae of the European corn borer. Probit analysis revealed comparable toxicity between wild-type crystals (isolated from B. thuringiensis subsp. kurstaki) and crystals produced from two spore-free mutants of the same subspecies. Death of the larvae was due to starvation, presumably through -endotoxin-induced gut paralysis. Inclusion bodies pretreated with -chymotrypsinogen were equally as toxic as native crystals for the insect larvae.