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Appl Environ Microbiol. 1981 September; 42(3): 408-412

Enhanced Production of Surfactin from Bacillus subtilis by Continuous Product Removal and Metal Cation Additions

D. G. Cooper, C. R. Macdonald, S. J. B. Duff and N. Kosaric

Biochemical Engineering, Faculty of Engineering Science, The University of Western Ontario, London, Ontario N6A 5B9, Canada

ABSTRACT

The lipopeptide, surfactin, is produced by Bacillus subtilis. A study has been made on large-scale production of this surfactant. A good yield was obtained from a glucose substrate fermentation by continuously removing the product by foam fractionation. The surfactin could be easily recovered from the collapsed foam by acid precipitation. The yield was also improved by the addition of either iron or manganese salts. Hydrocarbon addition to the medium, which normally increases biosurfactant production, completely inhibited surfactin production by B. subtilis.

Present address: McGill University, Department of Chemical Engineering, Montreal, Quebec H3A 2A7, Canada.

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