This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Counotte, G. H. M. Articles by DEBie, M. J. A. Search for Related Content PubMed PubMed Citation Articles by Counotte, G. H. M. Articles by DEBie, M. J. A. Agricola Articles by Counotte, G. H. M. Articles by DEBie, M. J. A.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1981 October; 42(4): 649-655

Role of Megasphaera elsdenii in the Fermentation of DL-[2-¹³C]lactate in the Rumen of Dairy Cattle

G. H. M. Counotte¹, R. A. Prins^1,, R. H. A. M. Janssen² and M. J. A. DEBie²

¹ Laboratory of Animal Nutrition, Zootechnical Department, State University of Utrecht, Utrecht, The Netherlands
² Laboratory of Organic Chemistry, State University of Utrecht, Utrecht, The Netherlands

ABSTRACT

Since Megasphaera elsdenii ferments a variable part of DL-lactate to butyrate, measurement of the percentage of DL-lactate fermented to propionate via the acrylate pathway in rumen contents will underestimate the participation of M. elsdenii in the DL-lactate fermentation. The percentage of DL-[2-¹³C]lactate fermented via the acrylate pathway and the percentage of DL-lactate fermented to butyrate can be measured with ¹³C-FT (Fourier transform)-nuclear magnetic resonance. On the average, the contribution of M. elsdenii to DL-lactate fermentation in the rumen of dairy cattle was found to be 74% (standard deviation, 13%), but differed with animal or diet. After feeding a cow readily fermentable carbohydrates, the contribution of M. elsdenii to the fermentation of DL-lactate increased as a consequence of catabolite repression in other DL-lactate-fermenting bacteria.

Present address: Department of Bacteriology, University of California, Davis, CA 95616.

This article has been cited by other articles:

• Bramley, E., Lean, I. J., Fulkerson, W. J., Stevenson, M. A., Rabiee, A. R., Costa, N. D. (2008). The Definition of Acidosis in Dairy Herds Predominantly Fed on Pasture and Concentrates. J DAIRY SCI 91: 308-321 [Abstract] [Full Text]  
• Belenguer, A., Duncan, S. H., Holtrop, G., Anderson, S. E., Lobley, G. E., Flint, H. J. (2007). Impact of pH on Lactate Formation and Utilization by Human Fecal Microbial Communities. Appl. Environ. Microbiol. 73: 6526-6533 [Abstract] [Full Text]  
• Nagaraja, T. G., Titgemeyer, E. C. (2007). Ruminal Acidosis in Beef Cattle: The Current Microbiological and Nutritional Outlook. J DAIRY SCI 90: E17-E38 [Abstract] [Full Text]  
• Suarez, B. J., Van Reenen, C. G., Stockhofe, N., Dijkstra, J., Gerrits, W. J. J. (2007). Effect of Roughage Source and Roughage to Concentrate Ratio on Animal Performance and Rumen Development in Veal Calves. J DAIRY SCI 90: 2390-2403 [Abstract] [Full Text]  
• Gozho, G. N., Krause, D. O., Plaizier, J. C. (2006). Rumen lipopolysaccharide and inflammation during grain adaptation and subacute ruminal acidosis in steers.. J DAIRY SCI 89: 4404-4413 [Abstract] [Full Text]  
• Miller, D. N., Berry, E. D., Wells, J. E., Ferrell, C. L., Archibeque, S. L., Freetly, H. C. (2006). Influence of genotype and diet on steer performance, manure odor, and carriage of pathogenic and other fecal bacteria. III. Odorous compound production. J ANIM SCI 84: 2533-2545 [Abstract] [Full Text]  
• Hristov, A. N., Kennington, L. R., McGuire, M. A., Hunt, C. W. (2005). Effect of diets containing linoleic acid- or oleic acid-rich oils on ruminal fermentation and nutrient digestibility, and performance and fatty acid composition of adipose and muscle tissues of finishing cattle. J ANIM SCI 83: 1312-1321 [Abstract] [Full Text]  
• Duncan, S. H., Louis, P., Flint, H. J. (2004). Lactate-Utilizing Bacteria, Isolated from Human Feces, That Produce Butyrate as a Major Fermentation Product. Appl. Environ. Microbiol. 70: 5810-5817 [Abstract] [Full Text]  
• Stanton, T. B., McDowall, J. S., Rasmussen, M. A. (2004). Diverse Tetracycline Resistance Genotypes of Megasphaera elsdenii Strains Selectively Cultured from Swine Feces. Appl. Environ. Microbiol. 70: 3754-3757 [Abstract] [Full Text]  
• Stanton, T. B., Humphrey, S. B. (2003). Isolation of Tetracycline-Resistant Megasphaera elsdenii Strains with Novel Mosaic Gene Combinations of tet(O) and tet(W) from Swine. Appl. Environ. Microbiol. 69: 3874-3882 [Abstract] [Full Text]  
• Krehbiel, C. R., Rust, S. R., Zhang, G., Gilliland, S. E. (2003). Bacterial direct-fed microbials in ruminant diets: Performance response and mode of action. J ANIM SCI 81: E120-132 [Abstract] [Full Text]