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Appl Environ Microbiol. 1981 October; 42(4): 672-676

Immobilization of Escherichia coli Cells Containing Aspartase Activity with Polyurethane and Its Application for L-Aspartic Acid Production

Murray C. Fusee, Wayne E. Swann and Gary J. Calton

1 Research Division, W. R. Grace & Co., Columbia, Maryland 21044

ABSTRACT

Whole cells of Escherichia coli containing aspartase activity were immobilized by mixing a cell suspension with a liquid isocyanate-capped polyurethane prepolymer (Hypol). The immobilized cell preparation was used to convert ammonium fumarate to L-aspartic acid. Properties of the immobilized E. coli cells containing aspartase were investigated with a batch reactor. A 1.67-fold increase in the L-aspartic acid production rate was observed at 37°C as compared to 25°C operating temperature. The pH optimum was broad, ranging from 8.5 to 9.2. Increasing the concentration of ammonium fumarate to 1.5 M from 1.0 M negatively affected the reaction rate. L-Aspartic acid was produced at an average rate of 2.18 x 10–4 mol/min per g (wet weight) of immobilized E. coli cells with a 37°C substrate solution consisting of 1.0 M ammonium fumarate with 1 mM Mg2+ (pH 9.0).

Appl Environ Microbiol. 1981 October; 42(4): 672-676




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