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Appl Environ Microbiol. 1981 November; 42(5): 897-903

Transduction of Lactose Metabolism by Streptococcus cremoris C3 Temperate Phage

¹ Department of Food Science and Nutrition, University of Minnesota, St. Paul, Minnesota 55108
² Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota 55108

ABSTRACT

Temperate phage was induced from Streptococcus cremoris C3 and morphologically characterized by high-resolution electron micrographic techniques. Interspecies genetic transfer of lactose-fermenting ability by the temperate phage was demonstrated, using two lactose-negative (Lac^–) S. lactis strains as recipients. Plasmid transfer was confirmed by agarose gel electrophoresis. Transductant plasmid profiles were of three types—those containing no visible plasmid deoxyribonucleic acid, those possessing a 23-megadalton (Mdal) plasmid, and those containing a 23-Mdal plasmid and a 30-Mdal plasmid. A Lac⁺ transductant could serve as a donor of the lac determinants during solid-surface matings. These results add to previously published reports of inter- and intraspecies genetic transfer in dairy starter cultures.

Scientific journal series paper no. 11760, Minnesota Agricultural Experiment Station, St. Paul, MN 55108.