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Appl Environ Microbiol. 1981 December; 42(6): 1119-1122

Isolation, Enumeration, and Maintenance of Rumen Anaerobic Fungi in Roll Tubes

Applied Biochemistry Division, Department of Scientific and Industrial Research, Palmerston North, New Zealand

ABSTRACT

Anaerobic phycomycetous fungi have been isolated from rumen fluid by using the Hungate roll tube technique. Cultures on solid substrates in roll tubes can be stored for long periods, and fungal zoospores can be enumerated in roll tubes.

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