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Appl Environ Microbiol. 1982 February; 43(2): 403-411

Factors Affecting Yield and Safety of Protein Production from Cassava by Cephalosporium eichhorniae

Y. Mikami, K. F. Gregory, W. L. Levadoux, C. Balagopalan and S. T. Whitwill^||

Department of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada

ABSTRACT

The properties of Cephalosporium eichhorniae 152 (ATCC 38255) affecting protein production from cassava carbohydrate, for use as an animal feed, were studied. This strain is a true thermophile, showing optimum growth at 45° to 47°C, maximum protein yield at 45°C, and no growth at 25°C. It has an optimum pH of about 3.8 and is obligately acidophilic, being unable to sustain growth at pH 6.0 and above in a liquid medium, or pH 7.0 and above on solid media. The optimum growth conditions of pH 3.8 and 45°C were strongly inhibitive to potential contaminants. It rapidly hydrolyzed cassava starch. It did not utilize sucrose, but some (around 16%) of the small sucrose component of cassava was chemically hydrolyzed during the process. Growth with cassava meal (50 g/liter [circa 45 g/liter, glucose equivalent]) was complete in around 20 h, yielding around 22.5 g/liter (dry biomass), containing 41% crude protein (48 to 50% crude protein in the mycelium) and 31% true protein (7.0 g/liter). Resting and germinating spores (10⁶ to 10⁸ per animal) injected by various routes into normal and -irradiated 6-week-old mice and 7-day-old chickens failed to initiate infections.

Present address: Research Institute for Chemobiodynamics, Chiba University, Chiba City, Japan.

Present address: Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

Present address: Central Tuber Crops Research Institute, Trivandrum 695017, Kerala, India.

^|| Present address: ENS Bio Logicals,% National Research Council of Canada, Ottawa K1A 0R6, Canada.

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