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Appl Environ Microbiol. 1982 February; 43(2): 483-485

Simplified Microassay for Pullulan Synthesis

M. A. J. Finkelman and A. Vardanis

1 Department of Microbiology and Immunology, The University of Western Ontario, and London Research Centre, Agriculture Canada, 2 London, Ontario N6A 5C1, Canada

ABSTRACT

A simple radiometric microassay for extracellular polysaccharide elaboration by yeast-like cells of Aureobasidium pullulans was developed, based upon a procedure originally described by Catley (FEBS Lett. 20:174-176, 1972). Incorporation of [14C]glucose into pullulan was linear with respect to time and cell dose. The pH and temperature optima for elaboration were 5.3 and 30°C, respectively. Polysaccharide elaboration declined linearly with culture age.

Appl Environ Microbiol. 1982 February; 43(2): 483-485






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